The IAEC approval will be obtained to conduct the
study. It is an animal study using Swiss albino male mice. The mice from
Central Animal House, PIMS will be used for this study. The aqueous extract of Curcumin longa  will be procured from a competent herbal drug
manufacturer with complete phyto chemical constituents furnished by the
manufacturer. Swiss albino male mice weighing 25 to 30 gm and of 60 to 90 days
old will be used in this study. This will be maintained in plastic cages under controlled

 lighting
conditions(12h light/12h dark regime),relatively humidity(50±5%) and temperature
(37±2ºC),fed with pellets and will be given ad
libitum access to water. Actophotometer and other instruments used in
Morpurgo method can be used in the Dept of Pharmacology for this study. A group
of 6 mice in each group will be taken.

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Haloperidol will be administered at the dosage of 1mg/kg of body weight/day/animal4 intraperitoneally for 15 days.
All behavioral and rigidity assessments will be done weekly after 24 hours of
the last dose. The 1st group of 6 mice will be treated with vehicle and rest of the groups
will be treated with haloperidol and vehicle. The 3rd, 4th and 5th groups of mice will be
pretreated with the aqueous extract of Curcumin
longa  50mg, 100mg and 200mg/kg of
body weight respectively as per the protocol listed below:

 

   GROUP 1:

 

          Control group with minimum of 6 mice
will be treated only with vehicle (sterile water)                   

         15 days

 

         GROUP 2:

 

           Control group with minimum of 6mice
treated with haloperidol and vehicle for

 

            15 days.

 

GROUP 3:

 

  Experimental group with a
minimum of 6 mice pretreated with the aqueous extract     of Curcumin
longa 50mg/kg of body weight /day/animal intraperitoneally for 15days. Haloperidol and vehicle will be
administered 50 mins after Curcumin longa
administration for 15 days.

 

GROUP 4:

 

Experimental group with a minimum of 6 mice pretreated with aqueous
extract of Curcumin longa 100 mg/kg
of body weight/day/animal intraperitoneally for 15 days, haloperidol and vehicle will be administered 50mins after Curcumin longa administration for 15
days.

 

GROUP 5:

 

Experimental group with a minimum of 6 mice
pretreated with aqueous extract of Curcumin
longa 200mg/kg of body weight/day/animal intraperitoneally for 15 days, haloperidol and vehicle will be administered
50 mins after Curcumin longa
administration for 15 days.

 

  The locomotor activity and
catatonia scoring of control and experimental animals must be measured by using
actophotometer and Morpurgo methods respectively on 8th and 15th day before drug administration.
The data observed will be tabulated for statistical analysis.

 

 

 

 

 

 

 

 

Methods of statistical analysis & test applied:

 

All the results will be expressed as mean ± SEM.
Data will be analyzed by one way analysis of variance (ANOVA) followed by
Tukey’s multiple comparison test using SPSS 11 software. p<0.05 will be considered as statistically significant.  

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