Sr. No Strain Indole MR VP Simmon Citrate Catalase Oxidase TSI                 H2S Acid Gas 1 LB 1. 1     +ve Weak +ve Positive -ve +ve -ve -ve 2 LB 2. 200   -ve -ve -ve -ve Positive +ve -ve -ve -ve 3 LB 3 200 -ve -ve -ve +ve Positive +ve -ve +ve -ve 4 LB 3. 7 -ve -ve -ve – ve Positive -ve +ve -ve -ve 5 LB 4.

2 -ve   -ve -ve Positive -ve -ve +ve _ve 6 LB 4. 7 +ve – -ve + ve Positive -ve +ve -ve -ve 7 LB 5  Big 1 -ve       Positive +ve -ve +ve -ve 8 LB Type 6 -ve -ve +ve -ve Positive +ve -ve +ve -ve 9 LB 8 -ve -ve -ve +ve Negative -ve -ve +ve -ve 10 LB 9 -ve       Positive -ve +ve -ve -ve 11 LB 10 -ve -ve +ve +ve Positive -ve -ve +ve -ve 12 LB 11 -ve -ve -ve -ve Negative -ve -ve -ve -ve 13 NA+LB 20 + ve -ve -ve +ve Positive -ve +ve -ve -ve 14 M9+ LB 14 -ve – ve + ve – ve Positive +ve -ve +ve -ve   Evaluation of Chromium Tolerance:Initialscreening was done at the time of isolation of isolates on 50 ppm. Out of 14isolates 7 isolates were selected on the basis of their ability to grow/tolerate at different concentrations of Chromium (100, 300, 500, 700, 900,1100, 1300 mg/L in LB broth medium at given time intervals. The growth of theisolates was determined by measuring the optical density of the cell suspensionat 600 nm at 24 h time interval using a spectrophotometer.Outof 14 isolates NA+LB and LB 4.

7 havesimilar results (krieg 1984). After comparingtheir results and visual appearance it was found that cultures are same.There aredifferent mechanisms of metal tolerance and reductionoftoxic metals.                              Table: Growth of NA+LB at different conc of K2Cr2O7   PCR Amplification and sequencing:16S sequencing results of NA+LB was obtained andsubjected to BlastN.

With query cover 96 %, NA+LB Showing 83% identity with Acinetobacter pitti   (Accession no. NR117621.1) accessionnumber provided for NA+LB (PL) was LC155825.1.    Fig.

Shows the phylogenetic tree of Acenetobactor pitti strain.  Antibiotic sensitivity Test:   Fig. Pattern of antibiotic sensitivity andResistance pattern of Acenetobactor pittistrain.  Sr. No Antibiotics Conc in mcg Zone of sensitivity in mm Aggregate of zone of sensitivity   Susceptibility 1 Amikacin (AK) 30 26 mm 24 mm  25 mm  25 R 2 Ampicillin (AMP) 10 10 mm 12 mm  13 mm  12 I 3 Aztreonam (AT) 30 19  mm  16 mm 17 mm  17   4 Chlorampenicol © 30 19 mm 20 mm 18 mm  18   5 Ceftazidime (CAZ) 30 18 mm  19 mm 17 mm  18   6 Ciprofloxacin (CIP) 5 27 mm 27 mm  25 mm 26   7 Co-Trimoxazole (COT) 25 14 mm 16 mm 14 mm  14   8 Ceftriaxone (CTR) 30 23 mm  21mm 23 mm  22   9 Cefuroxime (CXM)  30 12 mm 13 mm  14 mm  13   10 Fusidic Acid (FC) 30 Overlap       R 11 Gentamicin (G) 10 25 mm 23mm  26 mm  24   12 Imipenem (IPM) 10 14 mm 16 mm  14 mm  14   13 Linezolid (LZ) 30 overlap     R 14 Meropenam (M) 10 24 mm 22 mm  23 mm  23   15 Oflaxin (OF) 5 23 mm 25 mm   24 mm 24   16 Piperacillin (PI) 100 25 mm 22 mm  24 mm  23   17 Rifampicin (Rif) 5 15 mm 14 mm  14 mm  14   18 Tetracyclin (TE) 30 16 mm 15 mm 22 mm 17   19 Vancomycin (V) 30 12 mm 11 mm  12 mm  12      Discussion:Soils containingheavy metals are potential sources for identifying toxic-metal-resistantBacteria.

Inthis study, we have isolated chromium resistant bacterial strains from soilsamplescollected fromBacteria belongs togenus Acenetobacter are ubiquitous innature; they can easily found various types of in soil and water They are normal inhabitants of humanskin and are respiratory tract. They can cause infection in debilitatedpatients (SD. 1976)  A. pittii belongsto the Acinetobacter calcoaceticus-baumannii complex.

(Nemec A1 2011) We haveisolated this bacteria frim chromite mine area.

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