Materials Tryptic Soy Broth (TSB), Methanol, Chloroform, Glycine
Materials and MethodsMaterials and Animals: Soybean phosphatidylcholine (PC)was purchased from Lipoid, Inc. (Alabaster, AL, US). Cholesterol, Vancomycin hydrochlorid, 2-Iminothiolanehydrochloride (Traut’s reagent), Lysostaphin, L-Cystein, TritonX-100 and Sodium Chloride (NaCl) were purchased from Sigma Aldrich (St Louis, MO, USA). Bloodagar, Tryptic Soy Broth (TSB), Methanol, Chloroform, Glycine and AmmoniumSulfate were obtained from Merck,Germany. 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(polyethylene glycol)-2000 (DSPE-PEG(2000) Maleimide) and…