Microbiology is the learning of small size organisms which we don’t see by naked eyes includes bacteria, fungi, virus, and parasites.
All of that are ubiquitous and they found everywhere in the environment even household dust. The microorganisms have many benefit for us but they also cause symptoms in human called as infectious disease. The microbial diagnosis need in the treatment because the clinical presentation of patient isn’t enough information. Several decade ago, the diagnostic approach has been start from the basic strategies such as microbial isolation and culture, staining and microscopy until it has continued to the molecular and antibiotic test about 10 years ago. Todays, the diagnostic scheme doesn’t stop and still has the problem and some limitation that should be solve in the future. ” What would you do if you want to improve diagnostic ” is the challenged question of many microbiologists especially who work in the laboratory and they often found any problem. The important concept of microbial diagnostic includes sensitive, specific, and less-timing.
Recently, I read the research article about the successful development of yeast biosensor that will recognize binding between fungal-derived secreted peptides and engineered receptor, the red pigment synthesis gene is activated, and result for visible on paper base. This tools are low-cost, easy to maintenance and handling, cost effective, and operate in hands. Moreover, it is in the point of care testing because it can perform at the site of patient, has simple method and easy to interpret the results which doesn’t need specialized equipment and laboratory, and it can guide rapid therapeutic decision of treatment. However, it still some limitation such as contamination and storage period. Another research is similar that is the fabrication of genosensor for the detection of HCV virus in clinical sample which probe cDNA will coated on electrode nanoparticles. All of data that previously described, I has any idea about designing of new sensor tool that use the bioinformatics knowledge’s for function. My tool has pattern similar as at home pregnancy test and it’s names that DNA barcode dipstick test. After the collecting of supernatant cell lysate of clinical samples, the dipstick puts on it immediately.
The dipstick has application that connect with online database in computer and special machine. Computer will provide the sequence analysis and basic local alignment search tool or BLAST program. While, the connected machine composes the barcode of life database or BOLD system that is the reference library of DNA barcodes which it can be used to identify unknown specimens. The BOLD system will contain the conserved sequences of many pathogens includes 16S ribosomal RNA of bacteria, ITS2 region of fungi, and core protein gene of virus that cause same syndrome such as diarrhea, pneumonia, and meningitis. This tool can compare and determine the matched similarities percentage of high amount main pathogen, it will detect other microorganisms such as microbiota that may relate with infectious disease and show on the screen of dipstick test within 1 hours at the same time.
This tool will provide genetic profiling of isolated microorganism in term of genomic, transcriptomic, and proteomic information which it covers the virulence factor and antibiotic resistance gene data and it may determine the stage of infection or distinguish between primary and reinfection if it is continuously developed. Although, we can develop new technology test for facilitating with microbial diagnosis in the present but we still have some problem about biosafety and low-level of microbiologist performance. From that problem, I would like to have any idea for planning to create the questionnaire about technique, microbiology activity and diagnosis procedure, specific materials and equipment. This questionnaire are send to the clinical laboratories in each hospital or public health institutions to survey the risk and the number of laboratory-acquired infection. I will support that all of laboratories should be improve the biosafety prospective by planning the risk assessment and management programs to assess possible cause of infection in order to identify the occupational risks that associated with working of infectious agents and plan preventative technique and procedure for increasing the safe work and quality control. Moreover, the clinical laboratories should provide the personal training and checkup health of microbiologist every year for controlling the risk from personal errors. Todays, the severe infectious disease can overcome more than the past because good and rapid diagnostic approaches are continuously developed for solving the problem and reduce any limitation.
The development of diagnostic scheme will lead to the proper treatment of patient and increase the quality life of world population. Although, the number of LAIs will decline in the present but every microbiologist should usually think and recognize about biological hazards. Moreover, the validation of laboratory tests are important because the test must have high performance, provide correct interpret results, and it are widely accepted as called gold standard.