1.1.         Introduction

Climate change is a serious thread for recent world and Global warming caused for increasing CO2 day by day is one of the main matter of concern.

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Increasing of CO2 in atmosphere can be reduced through different physical, chemical and biological methods. Among these three types of CO2 sequestration methods, microbial bio-sequestration is the most important and interesting part. Atmospheric CO2 are bio-sequestrated by different bacteria and algae such as Bacillus maris?avi, Bacillus pumilus, Virgibacillus pantothenticus,Chlamydomonas reinhardtii ,Dunaliella terteolecta, Scenedesmus obliquus, Chlorella saccharophila, Chlorella ellipsoidea, Navicula pelliculosa ,Cyclotella sp. Ditylum brightwellii. Most of these microbes have Carbonic anhydrase enzyme and able to convert atmospheric CO2 into different mineral carbonates, such as calcium carbonate and magnesium carbonate1.These microbes are possibly found in costrel aria and capture atmospheric CO2 by carbonate precipitation. These microbes also can be found at trace amounts in other normal natural environments such as soil, plant surfaces, pond water surfaces and any other places where direct contacts of atmospheric CO2 are found.

Additionally, the existence of large volumes of easily accessible subsurface brine at the surface provides a potential high capacity route for the conversion of CO2 into geologically stable mineral carbonates. Subsurface saline aquifers may sequester CO2 in various forms. However, any ex-situ sequestration process will rely on an efficient conversion to mineral carbonates. The rate of the mineral trapping process is slow and serves as the major disadvantage of this technology. It has been suggested that pH has a significant effect on both conversion rates and on the specific species that are precipitated.

An investigation into the parameters, most importantly pH, that affect this conversion rate is warranted since the evolution of brine pH following a pH adjustment and during reaction with CO2 at various temperatures and pressures is highly variable. Some species of this type of bacteria that produce bio-films has effectiveness in Carbon dioxide sequestration.

As Increasing Carbon Dioxide (CO2) in the atmosphere increase the threats of global warming and global diversity loss, industrially production of Carbon dioxide gas sequestrating microbes and use them in industries which has high potential of CO2 gas emission is one of the available harmless and eco-friendly approach. The present study reports the isolation and identification CO2 sequestrating bacteria based on microbial intracellular carbonic anhydrase.

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